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Reversible Terminators for Primer Extension

Firebird has introduced the 3'-ONH2 reversible terminator to replace bulky 3'-OCH2N3 groups and their problematic enzymology and cleavage.

 

How do they work?

The 3'-ONH2 group is accepted by Firebird proprietary polymerases, and blocks further primer extension. It is cleaved in seconds in buffered aqueous sodium nitrite.                     

 

 

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What are they used for?

Firebird architectures use this terminator in SNP analysis, for example:

 

Polymorphisms (SNPs) are detected with low background by competing Firebird reversible terminators with irreversible terminators, even when non-SNPped DNA is present in abundance.