Reversible Terminators for Primer Extension

Firebird has introduced the 3'-ONH2 reversible terminator to replace bulky 3'-OCH2N3 groups and their problematic enzymology and cleavage.


How do they work?

The 3'-ONH2 group is accepted by Firebird proprietary polymerases, and blocks further primer extension. It is cleaved in seconds in buffered aqueous sodium nitrite.                     
















What are they used for?

Firebird architectures use this terminator in SNP analysis, for example:


Polymorphisms (SNPs) are detected with low background by competing Firebird reversible terminators with irreversible terminators, even when non-SNPped DNA is present in abundance.