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Reversible terminators with disulfide-linked tags

Firebird offers reversible terminators with a 3'-ONH2 (protected as the acetoxime) and a disulfide linker carrying a free amino group, to which a tag (fluorescent dye or other moiety) can be attached. The disulfide can be rapidly cleaved with phosphine or thiol reagents.

 

 

 

Thymine
TONH2-ST

1 µmoles $1750.00

 

 

 

Cytosine
CONH2-ST

1 µmoles $2000.00

 

Adenine
AONH2-ST

1 µmoles $2400.00

 

 

Guanine
GONH2-ST

1 µmoles $2500.00

 

Please inquire for bulk pricing.

 

For enzymatic incorporation, the oxime must be deprotected to the free 3'-ONH2 before use, which can be achieved in situ with buffered aqueous methoxylamine.

 

Not available for sequencing applications on certain machines and in certain jurisdictions.  Please inquire.