The Z:P pair is retained during PCR amplification, even when present as consecutive base pairs.
AEGIS-nested primers suppress noise in multiplex PCR and support 23-plex PCR to detect RNA viruses.
Oligonucleotides containing AEGIS bases are available. Please inquire.
Firebird also prepares libraries of AEGIS oligonucleotides.
SAMRS can be combined with AEGIS in oligos to get highly multiplexed and highly clean PCR, and isothermal amplification.
Yang et al., 2011; Yang et al., 2010; Glushakova et al., 2015a